Welcome to JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) June 10, 2025
Advanced Search
Volume 51 Issue 4
Jul.  2020
Turn off MathJax
Article Contents
Abstract
References
Related Articles
ZHOU Chen-yun, ZHU Xiao-xia, XU Zi-lin, et al. Contrast-enhanced Ultrasonography and Color Doppler Ultrasonography for Dynamic Assessment of Intracranial Changes in Early MCAO Rats Models[J]. Journal of Sichuan University (Medical Sciences), 2020, 51(4): 515-520. DOI: 10.12182/20200760603
Citation: ZHOU Chen-yun, ZHU Xiao-xia, XU Zi-lin, et al. Contrast-enhanced Ultrasonography and Color Doppler Ultrasonography for Dynamic Assessment of Intracranial Changes in Early MCAO Rats Models[J]. Journal of Sichuan University (Medical Sciences), 2020, 51(4): 515-520. DOI: 10.12182/20200760603
shu

Contrast-enhanced Ultrasonography and Color Doppler Ultrasonography for Dynamic Assessment of Intracranial Changes in Early MCAO Rats Models

  • 1.

    Department of Ultrasound, West China Hospital, Sichuan University, Chengdu 610041, China

  • 2.

    West China School of Medicine, Sichuan University, Chengdu 610041, China

  • 3.

    Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China

More Information
  • Corresponding author:

    LI Jin, E-mail: jimlijin76@126.com

  • Received Date: September 26, 2019
  • Revised Date: November 22, 2019
  • Available Online: July 20, 2020
  • Published Date: July 19, 2020
    Abstract
  •   Objective  To assess the value of contrast-enhanced ultrasound (CEUS) and color Doppler ultrasound (DUS) on hemodynamic changes and cerebral perfusion quantitative analyses in Sprague-Dawley (SD) rats with focal permanent ischemic stroke.
      Methods  Sixteen SD rats with thin skulls were subjected to establish middle cerebral artery occlusion (MCAO) model. CEUS images were performed before modeling (T0), immediately after modeling (5-15 min after modeling, T1), 3 h after modeling (T2), followed by the measurement of bilateral middle cerebral artery (MCA), anterior cerebral artery (ACA), posterior cerebral artery (PCA) using DUS. The peak systolic velocity (PSV), end-diastolic velocity (EDV) and mean velocity (MV) of these arteries were obtained. The brain time-intensity curve was taken as interest region of the whole right brain, and the quantitative parameters of CEUS were obtained, including peak intensity (PI), area under the curve (AUC), wash in slope (WIS), time to peak (TTP), rise time (RT) and time from peak to one half (TPH). The modified neurological deficit score (mNSS) of the rats was performed 3 h after the modeling, and the data of the rats with a score of 9-11 were statistically analyzed.
      Results   A total of 12 rats were successfully modeled and completed with mNSS score 9-11. No blood flow signals were observed on the right MCA and ACA in the 12 rats at T1 and T2. From T0 to T1, PI, AUC and WIS of the right hemisphere decreased sharply with TTP and RT significantly prolonged, and the differences were statistically significant. However, there was no significant difference in hemodynamic parameters at that period of time. From T1 to T2, there were no significant changes in CEUS quantitative parameters (except AUC and TPH), while PSV, EDV, MV of LMCA and bilateral PCA showed significant acceleration, and the differences were statistically significant.
      Conclusion  CEUS and DUS can reveal the intracranial hemodynamics and brain tissue perfusion trends of MCAO rats, which could be new methods in assessment of ischemic stroke model at multiple time points.
    • FullText(HTML)
    • References (21)
  • [1]
    RAFII M S, HILLIS A E. Compendium of cerebrovascular diseases. Int Rev Psychiatry,2006,18(5): 395–407. DOI: 10.1080/09540260600935405
    [2]
    JIN H, ZHU S, WEI J W, et al. Factors associated with prehospital delays in the presentation of acute stroke in urban China. Stroke,2012,43(2): 362–370. DOI: 10.1161/STROKEAHA.111.623512
    [3]
    HERSON P S, TRAYSTMAN R J. Animal models of stroke: translational potential at present and in 2050. Future Neurol,2014,9(5): 541–551. DOI: 10.2217/fnl.14.44
    [4]
    ZHANG L, ZHANG R L, JIANG Q, et al. Focal embolic cerebral ischemia in the rat. Nat Protoc,2015,10(4): 539–547. DOI: 10.1038/nprot.2015.036
    [5]
    RIDOLFI F, ABBATTISTA T, BUSILACCHI P, et al. Contrast-enhanced ultrasound evaluation of hepatic microvascular changes in liver diseases. World J Gastroenterol,2012,18(37): 5225–5230. DOI: doi:10.3748/wjg.v18.i37.5225
    [6]
    QUAIA E, NOCENTINI A, TORELLI L. Assessment of a new mathematical model for the computation of numerical parameters related to renal cortical blood flow and fractional blood volume by contrast-enhanced ultrasound. Ultrasound Med Biol,2009,35(4): 616–627. DOI: 10.1016/j.ultrasmedbio.2008.10.003
    [7]
    PORTER T R, XIE F. Myocardial perfusion imaging with contrast ultrasound. JACC Cardiovasc Imaging,2010,3(2): 176–187. DOI: 10.1016/j.jcmg.2009.09.024
    [8]
    WEBER M A, KRIX M, JAPPE U, et al. Pathologic skeletal muscle perfusion in patients with myositis: detection with quantitative contrast-enhanced US—initial results. Radiology,2006,238(2): 640–649. DOI: 10.1148/radiol.2382041822
    [9]
    CHRISTIANSEN J P, LEONG-POI H, AMISS L R, et al. Skin perfusion assessed by contrast ultrasound predicts tissue survival in a free flap model. Ultrasound Med Biol,2002,28(3): 315–320. DOI: 10.1016/S0301-5629(01)00523-3
    [10]
    KLAUSER A, DEMHARTER J, DE MARCHI A, et al. Contrast enhanced gray-scale sonography in assessment of joint vascularity in rheumatoid arthritis: results from the IACUS study group. Eur Radiol,2005,15(12): 2404–2410. DOI: 10.1007/s00330-005-2884-9
    [11]
    CHEN L, ZHAN W W, SHEN Z J, et al. Blood perfusion of the contralateral testis evaluated with contrast-enhanced ultrasound in rabbits with unilateral testicular torsion. Asian J Androl,2009,11(2): 253–260. DOI: 10.1038/aja.2008.13
    [12]
    KASPAR M, PARTOVI S, ASCHWANDEN M, et al. Assessment of microcirculation by contrast-enhanced ultrasound: a new approach in vascular medicine. Swiss Med Wkly, 2015,145:w14047[2019-09-08]. https://doi.org/10.4414/smw.2015.14047.
    [13]
    HWANG M, SRIDHARAN A, DARGE K, et al. Novel quantitative contrast-enhanced ultrasound detection of hypoxic ischemic injury in neonates and infants: pilot study 1. J Ultrasound Med,2019,38(8): 2025–2038. DOI: 10.1002/jum.14892
    [14]
    ERRICO C, PIERRE J, PEZET S, et al. Ultrafast ultrasound localization microscopy for deep super-resolution vascular imaging. Nature,2015,527(7579): 499–502. DOI: 10.1038/nature16066
    [15]
    URBAN A, MACE E, BRUNNER C, et al. Chronic assessment of cerebral hemodynamics during rat forepaw electrical stimulation using functional ultrasound imaging. Neuroimage,2014,101: 138–149. DOI: 10.1016/j.neuroimage.2014.06.063
    [16]
    LOURBOPOULOS A, KARACOSTAS D, ARTEMIS N, et al. Effectiveness of a new modified intraluminal suture for temporary middle cerebral artery occlusion in rats of various weight. J Neurosci Methods,2008,173(2): 225–234. DOI: 10.1016/j.jneumeth.2008.06.018
    [17]
    WANG C, LI F, GUAN Y, et al. Bone marrow stromal cells combined with oxiracetam influences the expression of B-cell lymphoma 2 in rats with ischemic stroke. J Stroke Cerebrovasc Dis,2014,23(10): 2591–2597. DOI: 10.1016/j.jstrokecerebrovasdis.2014.05.035
    [18]
    LAPERGUE B, DEROIDE N, POCARD M, et al. Transcranial duplex sonography for monitoring circle of Willis artery occlusion in a rat embolic stroke model. J Neurosci Methods,2011,197(2): 289–296. DOI: 10.1016/j.jneumeth.2011.02.016
    [19]
    FEDERLEIN J, POSTERT T, MEVES S, et al. Ultrasonic evaluation of pathological brain perfusion in acute stroke using second harmonic imaging. J Neurol Neurosurg Psychiatry,2000,69(5): 616–622. DOI: 10.1136/jnnp.69.5.616
    [20]
    SEIDEL G, ALBERS T, MEYER K, et al. Perfusion harmonic imaging in acute middle cerebral artery infarction. Ultrasound Med Biol,2003,29(9): 1245–1251. DOI: 10.1016/S0301-5629(03)01016-0
    [21]
    KIM J H, SHIN T, CHUNG J D, et al. Temporal pattern of blood volume change in cerebral infarction: evaluation with dynamic contrast-enhanced T2*-weighted MR imaging. AJR Am J Roentgenol,1998,170(3): 765–770. DOI: 10.2214/ajr.170.3.9490971
    • Relative Articles

  • Related Articles

    [1]QIN Yao, YIN Wan-hong, ZENG Xue-ying, WANG Jie, ZHU Yao, CCUSG  . Reflections on the Application of Critical Care Ultrasound in Viral Pneumonia Patients with Circulatory Dysfunction—New Classification and Precision Treatment Based on Ciritical Care Ultrasound[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2021, 52(4): 555-560. DOI: 10.12182/20210360502
    [2]LIU Ke-ling, PENG Wan-lin, XU Xu, LUO Ting, ZHANG Jin-ge, HU Si-xian, DIAO Kai-yue, LI Lei, XIA Chun-chao, LI Zhen-lin. The Application Value of One-stop CT Myocardial Perfusion Imaging in the Evaluation of Patients with Severe Coronary Artery Stenosis[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2019, 50(6): 878-883.
    [3]ZHOU Chen-yun, ZHU Xiao-xia, TANG Lin, LI Jia-wu, YAN Feng, LUO Yan. Application of Multimodal Transcranial Ultrasound in SD Rats of Cerebral Ischemic Model at Super Early Stage[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2019, 50(3): 344-349.
    [4]WANG Qing, FAN Yi-ding, YAN Lü-nan. et al. Clinical Research on the Post-operative Hemodynamics Changes in Donors after Living Donor Liver Transplantation[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2018, 49(6): 920-923.
    [5]ZHANG Jin-ge, LIU Ke-ling, ZHAO Li-hong. et al. Comparison of MR Perfusion Weighted Imaging and CT Perfusion Imaging in Assessing Hemodynamics of Patients with Moyamoya Disease[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2018, 49(5): 749-753.
    [6]YANG Fan, YANG Tai-zhu, TIAN Tian, WANG Jing-xin, LUO Hong. Perfusion Imaging of Ovarian Masses with Contrast-enhanced Ultrasonography[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2018, 49(4): 587-593.
    [7]ZHANG Ze-ming, LIU Li-jun, AN Lei. et al. Effects of Lung Ventilation Perfusion Imaging Examination on Pregnant Outcomes of Rabbits[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2016, 47(6): 866-868.
    [8]YANG Lu-lu, MA Lin, WANG Hong.et al. A Preliminary Study on Portal Vein Hemodynamic Changes and Liver Regeneration in Rats after 70% Partial Hepatectomy[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2016, 47(3): 326-330.
    [9]LIU Yong, ZHANG Er-yong, FANG Zhi, GU Jun, QIAN Hong. Mid-long-term Clinical Results and Hemodynamic Evaluation of Aortic Valve Replacement with 17 mm St. Jude Medical Regent Valve[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2013, 44(2): 316-318,326.
    [10]YANG Fan, YANG Tai-zhu, LUO Hong, LI Chang-xian, YAO Jin, YAN Xia-yu, SONG Bin. Comparative Study of Ultrasonography and Magnetic Resonance Imaging in Midline Structures of Fetal Brain[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2012, 43(5): 720-724.
    • Supplements (0)

  • Cited by

    Periodical cited type(2)

    1. 李祥龙，陈香州，雷围程，李立东，李尧，杨福兵，杨晓波. 建立与青年大鼠神经功能损伤程度相同的老年大鼠大脑中动脉阻塞模型. 西南医科大学学报. 2023(03): 213-216 .
    2. 娄惠娟，丛德毓，张红石，蒙蒙，李湛新，王宇峰. 局灶性脑缺血动物模型建立及评价概况. 吉林中医药. 2023(10): 1117-1122 .

    Other cited types(0)

Catalog

    Get Citation
    PDF
    XML
    Article views (3623) PDF downloads (75) Cited by(2)
    • Website Copyright © Editorial Office of Journal of Sichuan University (Medical Sciences).
    • 17, Section 3, Renmin Nanlu Road, Wuhou District, Chengdu 610041, People’s Republic of China
    • Tel：+86-028-85501320 +86-028-85500106       E-mail: scuxbyxb@scu.edu.cn

    Official website of Journal of Sichuan University (Medical Sciences)

    WeChat official account of Journal of Sichuan University(Medical Sciences)

    WeChat subscription account of Journal of Sichuan University(Medical Sciences)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return