Comparison Between Different Mitochondrial Staining Methods in Cell and Tissue Samples

LONG Dan; ZHOU Yan-ni; FENG Li; LI Sheng-fu

doi:10.12182/20200560205

Volume 51 Issue 3

May 2020

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JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) > 2020 > 51(3): 388-392. > DOI: 10.12182/20200560205

LONG Dan, ZHOU Yan-ni, FENG Li, et al. Comparison Between Different Mitochondrial Staining Methods in Cell and Tissue Samples[J]. Journal of Sichuan University (Medical Sciences), 2020, 51(3): 388-392. DOI: 10.12182/20200560205

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Comparison Between Different Mitochondrial Staining Methods in Cell and Tissue Samples

Key Laboratory of Transplant Engineering and Immunology of the Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China

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Corresponding author:
LI Sheng-fu, E-mail: lishengfu@126.com
Received Date: May 16, 2019
Revised Date: November 15, 2019
Available Online: May 24, 2020
Published Date: May 19, 2020

Abstract

Abstract

Objective To compare the effects of mitochondria staining between specific mitochondrial fluorescent probes and anti-mitochondrial protein antibody in cell and tissue samples.
Methods The HepG2 cells fixed by 4% paraformaldehyde were stained with MitoTracker Deep Red (100 nmol/L) or anti-Grp75 antibody (75 nmol/L or 100 nmol/L). The human healthy liver tissue samples fixed by 4% paraformaldehyde were stained with 150 nmol/L MitoTracker Deep Red or anti-Grp75 antibody. The above stained cell and tissue samples were observed using confocal microscopy.
Results We found non-specific staining in HeLa cells and obscure mitochondrial image using MitoTracker Deep Red probes, while clear tubular and punctate distribution using anti-Grp75 antibody. In contrast, we observed more specific and better effects of MitoTracker Deep Red probes-stained liver tissue samples as compared to the antibody.
Conclusion To visualize mitochondria, the anti-Grp75 antibody staining worked better on cells and the MitoTracker Deep Red probes are more suitable for tissue samples.
- Mitochondria,
- MitoTracker Deep Red,
- Grp75,
- Probe

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References

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