The Mechanism of Ciclosporin A Regulating the Expression of LOX-1 on Vascular Endothelium ofHyperlipidemic Rats

Objective To observe the influence of Ciclosporin A (CsA) on the expression of lectin-like oxidized low-density lipoprotein (ox-LDL) receptor-1(LOX-1) on vascular endothelium of hyperlipidemic rats. Methods Hyperlipidemic rats model as for the study objects firstly were established. Then the high 〔5 mg/（kg·d）〕 and low〔1 mg/（kg·d）〕 dose of CsA were used to stimulate these rats, while the rats of normal blood lipids as control. After the end of intervention, the protein expressions of LOX-1 and C-reactive protein (CRP) on vascular endothelium of thoracic aorta were observed by Western blot and immunohistochemistry respectively. The serum levels of ox-LDL and reactive oxygen species (ROS) were also examined. Results An obviously up-regulated expression of LOX-1 on vascular endothelium of hyperlipidemic rats treated with CsA was observed (P<0.05), but not the serum level of ox-LDL (P>0.05). The further study result implied that CsA could increase the expression of CRP on vascular endothelial cells and serum level of ROS (P<0.05). Conclusion CsA can significantly up-regulate the expression of LOX-1 on vascular endothelium of hyperlipidemic rats through not only ox-LDL/LOX-1 pathway but also ROS/CRP pathway, which could cause the probability of occurrence of atherosclerosis.