差相富集-免疫荧光原位杂交技术检测膀胱癌患者循环肿瘤细胞的初步研究

杨玉帛; 白云金; 唐寅等

差相富集-免疫荧光原位杂交技术检测膀胱癌患者循环肿瘤细胞的初步研究

Detection of Circulating Tumor Cells in Bladder Cancer by Subtraction Enrichment and Immunostaining-

摘要

摘要: 目的利用差相富集-免疫荧光原位杂交技术（subtraction enrichment and immunostaining-fluorescence in situ hybridization，SE-iFISH）检测膀胱癌患者外周血循环肿瘤细胞（circulating tumor cells，CTCs），并探究该检测结果与肿瘤临床特征的关系。方法收集2016年4~6月收治入院的16例膀胱癌患者（实验组）临床病理资料，其中男14例，女2例，年龄（66.88±8.97）岁。另取10例非肿瘤患者为对照（对照组），其中男7例，女3例，年龄（61.90±9.13）岁。取外周静脉血各6 mL，差相富集法收集外周血CTCs, 肿瘤细胞标志物-iFISH检测6 mL血中所有CTCs的数量及相关生物学特性，比较CTCs的数量与膀胱癌临床特征（有无血尿、肿瘤大小、数目、是否侵及肌层、原发肿瘤分期、淋巴结转移、肿瘤级别、肿瘤病理学类型等）的关系。结果实验组CTCs阳性检出率为43.75%（7/16），对照组CTCs阳性检出率为0%（0/10），两组阳性检出率差异有统计学意义（P=0.022 7）。实验组CTCs含量为0~29个，中位数为0个，对照组CTCs含量为0个，两组CTCs含量差异有统计学意义（P=0.028 3）。实验组中，有无血尿、肿瘤大小、数目、是否侵及肌层、原发肿瘤分期、淋巴结转移、肿瘤级别、肿瘤病理学类型等亚组之间，CTCs阳性检出率的差异无统计学意义。结论SE-iFISH检测膀胱癌外周血CTCs可行性好。关于CTCs含量与膀胱癌患者临床特征的关系尚不肯定，需更大样本量予以说明。

Abstract: Objective To detect circulating tumor cells (CTCs) in peripheral blood from the patients with bladder cancer using subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH) and to explore the relation between CTCs detection result and the clinicpathological parameters of tumors. Methods Sixteen patients 〔(66.88±8.97) yr.〕 with bladder cancerwere included, whlile 10 normal people 〔(61.90±9.13) yr.〕 were enrolled as control. Peripheral blood was harvested (6 mL per case) for the detection of CTCs with the method of subtraction enrichment and SE-iFISH, and the correlations of CTCs level to the clinicpathological parameters of tumors were analyzed, including hematuria, size, number, NMIBC or MIBC, stage, grade and pathological type. Results Positive rates of CTCs in experimental and control group were 43.75% (7/16) and 0% (0/10), respectively. The difference was statistically significant (P=0.022 7). The number of CTCS was not associated with the clinicpathological parameters of bladder cancer, which probably due to small sample size. Conclusion It is applicable to detect CTCs of bladder cancer by SE-iFISH.

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