Abstract: ObjectiveTo determine the effect of testosterone on serum glucose, lipid, uric acid and insulin metabolism in male patients with type 2 diabetes mellitus. Methods A total of 205 male patients with type 2 diabetes participated in this study. They were divided into two groups: those with normal testosterone (TT) （TT≥12 nmol/L, n=135） and those with low TT （TT<12 nmol/L, n=70). Their body mass, waist circumference (WC), body mass index (BMI), blood glucose,total cholesterol (TC),triglycerides (TG),serum uric acid (SUA), insulin, testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2), and sex hormone binding globulin (SHBG) were measured. The insulin resistance index (HOMA-IR) of the participants was calculated using a homeostasis model. Sex hormone levels were compared across the four groups divided by HOMA-IR and SUA in quartiles. ResultsThe participants with low TT had higher age, SUA, BMI, WC, and HOMA-IR (P<0.05). TT and SHBG decreased with increased HOMA-IR index (P<0.05). TT, LH, FSH and SHBG decreased with increased SUA (P<0.05). The logistic regression model showed that SUA and BMI were predictors of hypogonadism. Conclusion Male patients with type 2 diabetes who are prone to hypogonadism,are possibly related to increased SUA and obesity.