Abstract: Objective To investigate the relationship of human papillomavirus (HPV) subtypes and multiple infections with different cervical precancerous diseases. Methods Retrospective study was done to review 1 226 patients with different cervical lesions who were pathologically diagnosed and scanned for HPV 23 subtypes with positive results from June 2006 to May 2012. These patients were divided into the following groups, chronic cervicitis, cervical condyloma, cervical intraepithelium neoplasia grade Ⅰ（CINⅠ） , grade Ⅱ(CINⅡ)，gradeⅢ (CINⅢ). Results There were significant differences in the proportion of HPV low risk types and high risk types between cervicitis, condyloma, CINⅠgroup and CINⅡ+Ⅲ groups （P<0.05）. HPV low risk types in condyloma group were mainly 6 and 11 subtype, while the other four groups were 42 and 43 subtype. The four most prevalence high risk types were 58，16，52，18 subtype. The infection rates of HPV16 were significant different in cervicitis（11.0%）, CINⅡ（20.3%）, and CINⅢ （20.2%）(P<0.01), and the infection rates of HPV58 was quite different between cervicitis （15.9%） and CINⅡ（21.4%）(P<0.05). HPV multiple infection rate in condyloma （68.8%） was significant different from that of cervicitis （23.1%）, CINI （26.1%）, CINⅡ（27.8%）and CINⅢ (27.1%)（P<0.01）；while the rest four groups were not significantly different （P>0.05）. Conclusion There is a unique epidemiologic characteristic of HPV infection in Sichuan Province. The HPV low risk types were mainly 42 and 43, and high risk types were mainly 58,16,52,18. It seems that HPV multiple infection is not the leading cause of progression of cervical disease.