Abstract: Objective To study morphological feature, biochemical characteristic and antibiotic resistance of Robinsoniella peoriensis (R.peoriensis) strain. Methods The clinical R.peoriensis strain was isolated from ananus swab samples being screened in ICU. Biochemical characteristic of the strain was completed by fully automatic microbial identification and drug susceptibility analysis system （BioMérieux，Marcy-l′E toile, France） with the ANC VitekⅡ. Antibiotic susceptibility in vitro was performed using agar dilution. Results The organism was found to be positive to saccharose, beta-galactopyr anosidase indoxyl, alpha-arabinosidase and so on, while negative to the others. The susceptibility test in vitro showed that this strain was resistant to clindamycin, rifampicin, moxifloxacin，while sensitive to vancomycin, metronidazole and tetracycline. Conclusion R.peoriensis is positive to many biochemical products such as saccharose. The clinical isolate of R.peoriensis strain is resistant to clindamycin, rifampicin and moxifloxacin.