PEGPU 3-D多孔支架在膀胱组织工程中的初步应用评价

栏目: 论著

Preliminary Evaluation of Biodegradable Scaffold of Polyethylene (PEGPU) Glycol Polyurethane in Bladder Tissue Engineering

1. Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China;
2. Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China

摘要: 【摘要】目的探讨膀胱上皮细胞、平滑肌细胞在聚乙二醇聚氨酯(PEGPU)3-D多孔支架的生长增殖能力,对PEGPU 3-D支架在膀胱组织工程中的潜在应用进行评价。方法鼠成纤维细胞接种于乳液固含量(m/m)为8%、12%、16%、20%(即孔径为90.8、76.7、40.9和39.6μm)的PEGPU 3-D聚氨酯多孔支架后,采用异硫氰酸荧光素(FITC)与4’,6-二脒基-2-苯基吲哚(DAPI)免疫荧光共染观察支架表面与支架内部细胞生长情况。采用免疫组织化学染色(检测α-actin与f-actin的表达)鉴定原代培养人膀胱平滑肌细胞。将人膀胱平滑肌细胞、上皮细胞和鼠成纤维细胞接种于PEGPU 3-D聚氨酯多孔支架后,采用胆囊收缩素八肽(CCK-8)检测法绘制细胞生长曲线。结果鼠成纤维细胞可在PEGPU 3-D多孔支架粘附、增殖,在平均孔径为40.9μm的3-D多孔支架上鼠成纤维细胞可获得最佳的生长、增殖状态。膀胱平滑肌细胞在平均孔径为90.8μm的3-D支架生长状态最佳,膀胱上皮细胞在平均孔径为39.6μm的3-D支架生长状态最佳。结论 3-D多孔PEGPU支架可支持膀胱上皮细胞与平滑肌细胞生长、增殖,具有膀胱组织工程应用的潜力。
- 组织工程 /
- 膀胱 /
- 生物材料
Abstract: 【Abstract】
- Tissue engineering /
- Bladder /
- Biological materials

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