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陈小菊, 程德云, 樊莉莉. 低氧对大鼠肺动脉平滑肌细胞和肺微血管内皮细胞表达及分泌fractalkine的影响[J]. 四川大学学报(医学版), 2012, 43(5): 661-665.
引用本文: 陈小菊, 程德云, 樊莉莉. 低氧对大鼠肺动脉平滑肌细胞和肺微血管内皮细胞表达及分泌fractalkine的影响[J]. 四川大学学报(医学版), 2012, 43(5): 661-665.
CHEN Xiao-ju, CHENG De-yun, FAN Li-li. Effect of Hypoxia on the Expression and Production of Fractalkine in Pulmonary Artery Smooth Muscle Cells and Pulmonary Microvascular Endothelial Cells[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(5): 661-665.
Citation: CHEN Xiao-ju, CHENG De-yun, FAN Li-li. Effect of Hypoxia on the Expression and Production of Fractalkine in Pulmonary Artery Smooth Muscle Cells and Pulmonary Microvascular Endothelial Cells[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(5): 661-665.

低氧对大鼠肺动脉平滑肌细胞和肺微血管内皮细胞表达及分泌fractalkine的影响

Effect of Hypoxia on the Expression and Production of Fractalkine in Pulmonary Artery Smooth Muscle Cells and Pulmonary Microvascular Endothelial Cells

  • 摘要: 目的 观察低氧对培养的大鼠肺动脉平滑肌细胞和肺微血管内皮细胞表达及分泌fractalkine(FKN)的影响。 方法 低氧处理体外培养的大鼠肺动脉平滑肌细胞和肺微血管内皮细胞12 h、24 h和48 h,采用原位杂交和免疫组化法检测细胞中FKN mRNA和蛋白的表达,酶联免疫法检测细胞上清液中FKN的浓度。 结果 ①与对照组比较,大鼠肺动脉平滑肌细胞在低氧处理12 h后,其FKN mRNA和蛋白表达、细胞上清液中FKN浓度无明显变化(P>0.05),低氧24 h后FKN mRNA和蛋白表达、细胞上清液中FKN浓度均增加(P<0.05),48 h后其增加则更加明显(P<0.01)。②大鼠肺微血管内皮细胞在低氧处理12 h、24 h和48 h后,其FKN mRNA和蛋白表达及细胞上清液中FKN浓度与对照组比较均无明显变化(P>0.05)。 结论 低氧刺激增加了大鼠肺动脉平滑肌细胞表达和分泌FKN。

     

    Abstract: Objective To investigate the effect of hypoxia on the expression and production of fractalkine (FKN) in cultured rat pulmonary artery smooth muscle cells (PASMCs) and pulmonary microvascular endothelial cells (PMVECs). Methods PASMCs and PMVECs from SD rat were cultured in vitro,and were exposed to hypoxia for 12 h,24 h and 48 h. The expressions of fractalkine mRNA and protein in PASMCs and PMVECs were measured by the methods of in situ hybridization and immunohistochemistry. The fractalkine concentrations in supernatant fluid of cultured PASMCs and PMVECs were measured by enzyme-linked immunosorbent assay. Results ① Compared with the control group, the expression and production of fractalkine in PASMCs did not increase after the treatment of hypoxia for 12 hours (P>0.05), but increased after being treated with hypoxia for 24 hours (P<0.05), and became more significant after 48 hours (P<0.01). ②Compared with the control group, there were no differences of FKN concentrations in supernatant fluid of PMVECs, FKN mRNA and protein levels in PMVECs after being treated with hypoxia for 12 hours, 24 hours or 48 hours (P>0.05). Conclusion Hypoxia stimulates the synthesis and secretion of fractalkine in cultured rat PASMCs.

     

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