欢迎来到《四川大学学报(医学版)》
陶若琳, 张水军, 郭文治, 等. 液-液相分离在肿瘤中作用的研究新进展[J]. 四川大学学报(医学版), 2024, 55(1): 24-30. DOI: 10.12182/20240160503
引用本文: 陶若琳, 张水军, 郭文治, 等. 液-液相分离在肿瘤中作用的研究新进展[J]. 四川大学学报(医学版), 2024, 55(1): 24-30. DOI: 10.12182/20240160503
TAO Ruolin, ZHANG Shuijun, GUO Wenzhi, et al. Research Progress in the Role of Liquid-Liquid Phase Separation in Human Cancer[J]. Journal of Sichuan University (Medical Sciences), 2024, 55(1): 24-30. DOI: 10.12182/20240160503
Citation: TAO Ruolin, ZHANG Shuijun, GUO Wenzhi, et al. Research Progress in the Role of Liquid-Liquid Phase Separation in Human Cancer[J]. Journal of Sichuan University (Medical Sciences), 2024, 55(1): 24-30. DOI: 10.12182/20240160503

液-液相分离在肿瘤中作用的研究新进展

Research Progress in the Role of Liquid-Liquid Phase Separation in Human Cancer

  • 摘要: 液-液相分离 (liquid-liquid phase separation, LLPS) 是指细胞蛋白质和核酸等生物大分子在弱多价相互作用的驱动下,凝聚成液态无膜细胞器的可逆过程。目前检测生物大分子相分离的方法主要包括光漂白后荧光恢复法等。异常LLPS与人类多种癌症发生发展相关,肿瘤相关生物大分子(mRNA、lncRNA、肿瘤相关蛋白等)可通过发生相分离或异常相分离,影响转录翻译和DNA损伤修复、调控细胞自噬和铁死亡功能,进而调控多种肿瘤的发生发展。本文总结了LLPS在肿瘤发生发展中作用机制的最新研究进展,阐述了包括mRNA、lncRNA、蛋白质等生物分子发生异常相分离后促进/抑制自噬、肿瘤免疫、DNA损伤修复、细胞铁死亡等,进而影响肿瘤发生发展。目前研究表明,多种生物大分子能够发生相分离调控转录翻译、表达、转录后修饰、细胞信号转导等生物学过程。基于此,拓展相分离研究领域,深入研究其分子机制和调控过程具有深远的科学前景。

     

    Abstract: Liquid-liquid phase separation (LLPS) is a reversible process, during which biological macromolecules, including proteins and nucleic acids, condense into liquid membraneless organelles under the influence of weak multivalent interactions. Currently, fluorescence recovery after photobleaching is the primary method used to detect the phase separation of biological macromolecules. Recent studies have revealed the link between abnormal LLPS and the pathogenesis and development of various human cancers. Through phase separation or abnormal phase separation, tumor-related biological macromolecules, such as mRNA, long noncoding RNAs (lncRNAs), and tumor-related proteins, can affect transcriptional translation and DNA damage repair, regulate the autophagy and ferroptosis functions of cells, and thus regulate the development of various tumors. In this review, we summarized the latest research findings on the mechanism of LLPS in the pathogenesis and progression of tumors and elaborated on the promotion or inhibition of autophagy, tumor immunity, DNA damage repair, and cell ferroptosis after abnormal phase separation of biomolecules, including mRNA, lncRNA, and proteins, which subsequently affects the pathogenesis and progression of tumors. According to published findings, many biological macromolecules can regulate transcriptional translation, expression, post-transcriptional modification, cell signal transduction, and other biological processes through phase separation. Therefore, further expansion of the research field of phase separation and in-depth investigation of its molecular mechanisms and regulatory processes hold extensive research potential.

     

/

返回文章
返回