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以SPF级早产猪建立肺发育模型的初步实验研究

王秋 周丹 谢亮 石芳 张伟坚 伏洪玲 刘瀚旻 丁玉春

王秋, 周丹, 谢亮, 等. 以SPF级早产猪建立肺发育模型的初步实验研究[J]. 四川大学学报(医学版), 2021, 52(5): 789-793. doi: 10.12182/20210960504
引用本文: 王秋, 周丹, 谢亮, 等. 以SPF级早产猪建立肺发育模型的初步实验研究[J]. 四川大学学报(医学版), 2021, 52(5): 789-793. doi: 10.12182/20210960504
WANG Qiu, ZHOU Dan, XIE Liang, et al. Preliminary Experimental Study of Establishing a Lung Development Model with Specific Pathogen Free Preterm Pigs[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION), 2021, 52(5): 789-793. doi: 10.12182/20210960504
Citation: WANG Qiu, ZHOU Dan, XIE Liang, et al. Preliminary Experimental Study of Establishing a Lung Development Model with Specific Pathogen Free Preterm Pigs[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION), 2021, 52(5): 789-793. doi: 10.12182/20210960504

以SPF级早产猪建立肺发育模型的初步实验研究

doi: 10.12182/20210960504
基金项目: 国家自然科学基金(No. 81941007)和四川省科技计划重点研发项目(No. 2019YFS0037)资助
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    E-mail:hanmin@vip.163.com

Preliminary Experimental Study of Establishing a Lung Development Model with Specific Pathogen Free Preterm Pigs

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  • 摘要:   目的  探究在排除高氧、机械通气、药物等医学干预的条件下,建立无特定病原体(specific pathogen free, SPF)级巴马早产猪肺发育动物模型的最佳剖宫产时间。  方法  选择胎龄113 天(gestational days, GD)、GD107、GD104、GD101和GD98的SPF级巴马猪系母猪分别进行剖宫产,观察早产仔猪存活情况。根据早产猪的一般资料、存活情况和肺石蜡切片HE染色观察结果,寻找最佳的建立SPF级早产猪肺发育模型的剖宫产时间。  结果  剖宫产7只母猪,共获取仔猪55只,其中产后3 h仍存活的共25只。GD98和GD101的仔猪全部死亡,GD104的仔猪存活7只,存活率为33.33%(7/21),GD107和GD113的仔猪全部存活。GD98的仔猪已具备完整的外形和肺部形状;GD104仔猪的肺部开放度已较大,薄壁型肺泡密度更高;GD107仔猪的肺基本处于开放状态,薄壁肺泡密度基本与正常足月猪相同。  结论  本研究提示可以利用无医疗干预的GD104 SPF级早产猪建立SPF级早产猪肺发育模型。
  • 图  1  GD98和GD113仔猪的肺部外形

    Figure  1.  External appearance of the lungs of GD98 and GD113 piglets

    图  2  不同胎龄肺组织在低倍镜下的观察结果。HE染色 ×40

    Figure  2.  Observation of lung tissue of different gestational ages at low magnification. HE staining ×40

    A: GD98; B: GD101; C: GD104; D:GD107; E: GD113.

    图  3  不同胎龄肺组织在高倍镜下的观察结果。HE染色 ×200

    Figure  3.  Observation of lung tissue of different gestational ages at high magnification. HE staining ×200

    A: GD98; B: GD101; C: GD104; D:GD107; E: GD113.

    表  1  各组仔猪一般情况

    Table  1.   General information of the piglets in each group

    GroupnLive piglets/case (%)Body mass/gGender (male/female)
    GD98 5 0 (0) 240±29.15 1/4
    GD101 12 0 (0) 234±53.51 4/8
    GD104 21 7 (33.3) 391±90.91 9/12
    GD107 5 5 (100) 546±51.77 2/3
    GD113 12 12 (100) 615±101.22 8/4
     GD: Age in gestational days.
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  • [1] VOYNOW J A. “New” bronchopulmonary dysplasia and chronic lung disease. Paediatr Respir Rev,2017,24(1): 17–18. doi: 10.1016/j.prrv.2017.06.006
    [2] 刘瀚旻, 陆权. 生命早期起源的慢性阻塞性肺病之研究. 中华儿科杂志,2019,57(4): 244–247. doi: 10.3760/cma.j.issn.0578-1310.2019.04.003
    [3] NARDIELLO C, MIŽÍKOVÁ I, MORTY R E, et al. Looking ahead: Where to next for animal models of bronchopulmonary dysplasia? Cell Tissue Res,2017,367(3): 457–468. doi: 10.1007/s00441-016-2534-3
    [4] CAMINITA F, MERWE M, HANCE F, et al. A preterm pig model of lung immaturity and spontaneous infant respiratory distress syndrome. Am J Physiol Lung Cell Mol Physiol,2015,308(2): 118–129. doi: 10.1152/ajplung.00173.2014
    [5] BUDDINGTON R K, SANGILD P T, HANCE B, et al. Prenatal gastrointestinal development in the pig and responses after preterm birth. J Anim Sci,2012,90(Suppl 4): 290–298. doi: 10.2527/jas.54604
    [6] ODLE J, LIN X, JACOBI S K, et al. The suckling piglet as an agrimedical model for the study of pediatric nutrition and metabolism. Annu Rev Anim Biosci,2014,2(1): 419–444. doi: 10.1146/annurev-animal-022513-114158
    [7] SANGILD P T, THYMANN T, SCHMIDT M, et al. Invited review: The preterm pig as a model in pediatric gastroenterology. J Anim Sci,2013,91(10): 4713–4729. doi: 10.2527/jas.2013-6359
    [8] 邹迪莎, 于健. 巴马小型猪动物模型在医学领域的研究进展. 中国畜牧兽医,2017,44(4): 1128–1134.
    [9] 高彩霞, 辛畅, 陈洪岩. 畜禽疫病防控用SPF猪的开发. 实验动物与比较医学,2018,38(4): 245–249. doi: 10.3969/j.issn.1674-5817.2018.04.001
    [10] MCPHERSON R L, JI F, WU G, et al. Growth and compositional changes of fetal tissues in pigs. J Anim Sci,2004,82(9): 2534–2540. doi: 10.2527/2004.8292534x
    [11] SZPINDA M, SIEDLACZEK W, SZPINDA A, et al. Volumetric growth of the lungs in human fetuses: an anatomical, hydrostatic and statistical study. Surg Radio Anat,2014,36(8): 813–820. doi: 10.1007/s00276-014-1269-7
    [12] PAEPE M E D, FRIEDMAN R M, GUNDOGAN F, et al. Postmortem lung weight/body weight standards for term and preterm infants. Pediatr Pulmonol,2005,40(5): 445–448. doi: 10.1002/ppul.20218
    [13] EIBY Y A, WRIGHT L L, KALANJATI V P, et al. A pig model of the preterm neonate: anthropometric and physiological characteristics. PLoS One,2013,8(7): e68763[2021-08-11]. https://doi.org/10.1371/journal.pone.0068763. doi: 10.1371/journal.pone.0068763
    [14] HIGUCHI M, HIRANO H, MAKI M. The thromboplastic activity of lung surfactant in amniotic fluid and its application to prenatal assessment of fetal lung maturity. Tohoku J Exp Med,1981,133(3): 267–273. doi: 10.1620/tjem.133.267
    [15] MIURA T. Models of lung branching morphogenesis. J Biochem,2015,157(3): 121–127. doi: 10.1093/jb/mvu087
    [16] MORTY R E. Recent advances in the pathogenesis of BPD. Semin Perinatol,2018,42(7): 404–412. doi: 10.1053/j.semperi.2018.09.001
    [17] JENSEN E A, SCHMIDT B. Epidemiology of bronchopulmonary dysplasia. Birth Defects Res,2014,100(3): 145–157. doi: 10.1002/bdra.23235
    [18] JOBE A H. Mechanisms of lung injury and bronchopulmonary dysplasia. Am J Perinatol,2016,33(11): 1076–1078. doi: 10.1055/s-0036-1586107
    [19] SILVA D M, NARDIELLO C, POZARSKA A, et al. Recent advances in the mechanisms of lung al-veolarization and the pathogenesis of bronchopulmonary dysplasia. Am J Physiol Lung Cell Mol Physiol,2015,309(11): 1239–1272. doi: 10.1152/ajplung.00268.2015
    [20] NIEDERMAIER S, HILGENDORFF A. Bronchopulmonary dysplasia: An overview about pathophysiologic concepts. Mol Cell Pediatr,2015,2(1): 2–9. doi: 10.1186/s40348-015-0013-7
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出版历程
  • 收稿日期:  2021-05-12
  • 修回日期:  2021-08-12
  • 网络出版日期:  2021-12-06
  • 刊出日期:  2021-09-20

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