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Volume 51 Issue 6
Nov.  2020
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WANG Zheng, ZHOU Xue-dong, REN Biao. Ergosterol Pathway of Candida albicans Promotes the Growth and Cariogenic Virulence of Streptococcus mutans[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION), 2020, 51(6): 742-748. doi: 10.12182/20201160203
Citation: WANG Zheng, ZHOU Xue-dong, REN Biao. Ergosterol Pathway of Candida albicans Promotes the Growth and Cariogenic Virulence of Streptococcus mutans[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION), 2020, 51(6): 742-748. doi: 10.12182/20201160203

Ergosterol Pathway of Candida albicans Promotes the Growth and Cariogenic Virulence of Streptococcus mutans

doi: 10.12182/20201160203
  • Received Date: 2020-06-22
  • Rev Recd Date: 2020-10-12
  • Publish Date: 2020-11-20
  •   Objective   To investigate the effect of Candida albicans (C. albicans) on proliferation and virulence of Streptococcus mutans (S. mutans), and to verify the role of the ergosterol pathway in it.  Methods  After single- and co-cultivation of C. albicans wild-type 5314 and S. mutans UA159, the absorbance value of OD600 and colony-forming units (CFU) were detected to reflect the influence of C. albicans on the growth of S. mutans. To reflect the influence of C. albicans on the virulence of S. mutans, the production of extracellular polysaccharides was detected by anthrone-vitriol method, and the acid production capacity was detected by a pH meter. After single- and co-cultivation of C. albicans mutant strains and S. mutans, the growth of S. mutans was evaluated by CFU. After inhibiting the ergosterol pathway by 0 mg/L, 0.012 5 mg/L, and 0.025 mg/L fluconazole for 24 h, the CFU of single- and co-cultivated C. albicans wild-type and S. mutans were detected.  Results  The OD600 absorbance value and CFU in theco-cultivation of C. albicans wild-type and S. mutans was higher than that in the single culture (P<0.05), and the production of extracellular polysaccharides in S. mutans was increased when S. mutans was co-cultured with C. albicans (P<0.05), accompanied with a more obvious decrease of pH (P<0.05). Fourteen strains in whole 42 C. albicans mutant strains lost the growth-promoting effect on S. mutans, including 6 ergosterol synthesis-related mutant strains. After co-cultivation of the 6 ergosterol synthesis-related mutant strains and S. mutans, the CFU of S. mutans remained unchanged or decreased. After inhibiting the ergosterol pathway by 0.012 5 mg/L and 0.025 mg/L fluconazole, the CFU of S. mutans in the co-cultivation of C. albicans wild-type and S. mutans was lower than that without fluconazole treatment (P<0.05), while the CFU of C. albicans and S. mutans single-cultivations did not change significantly (P>0.05) and the CFU of C. albicans in the co-cultivation of C. albicans wild-type and S. mutans did not change significantly (P>0.05).  Conclusion  C.albicans can enhance the growth ability and virulence of S. mutans through the ergosterol-related pathway. This process can be inhibited by fluconazole, which is expected to become a novel strategy to prevent and treat dental caries.
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  • [1]
    BAWASKAR H S, BAWASKAR P H. Oral diseases: a global public health challenge. Lancet,2020,395(10219): 185–186.
    [2]
    LAMONT R J, KOO H, HAJISHENGALLIS G. The oral microbiota: dynamic communities and host interactions. Nat Rev Microbiol,2018,6(12): 745–759.
    [3]
    CHOW K F. A review of excessive sugar metabolism on oral and general health. Chin J Dent Res,2017,20(4): 193–198.
    [4]
    SCANNAPIECO F A, CANTOS A. Oral inflammation and infection, and chronic medical diseases: implications for the elderly. Periodontol 2000,2016,72(1): 153–175.
    [5]
    QUAN J K, WANG X Z, SUN X Y, et al. Permanent teeth caries status of 12- to 15-year-olds in China: findings from the 4th national oral health survey. Chin J Dent Res,2018,21(3): 181–193.
    [6]
    周学东, 程磊, 郑黎薇. 全生命周期的龋病管理. 中华口腔医学杂志,2018,53(6): 367–373. doi: 10.3760/cma.j.issn.1002-0098.2018.06.002
    [7]
    ABUSLEME L, DUPUY A K, DUTZAN N, et al. The subgingival microbiome in health and periodontitis and its relationship with community biomass and inflammation. Isme J,2013,7(5): 1016–1025.
    [8]
    LEMOS JA, PALMER SR, ZENG L, et al. The biology of Streptococcus mutans.Microbiol Spectr, 2019, 7(1): 10.1128/microbiolspec.GPP3-0051-2018[2020-04-01].https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6615571/. doi: 10.1128/microbiolspec.GPP3-0051-2018.
    [9]
    李蓝, 张博文, 李继遥. 变异链球菌毒力相关转录调节因子的研究进展. 华西口腔医学杂志,2016,34(6): 643–646. doi: 10.7518/hxkq.2016.06.018
    [10]
    KRZYŚCIAK W, JURCZAK A, KOŚCIELNIAK D, et al. The virulence of Streptococcus mutans and the ability to form biofilms. Eur J Clin Microbiol Infect Dis,2014,33(4): 499–515.
    [11]
    SINGH A, VERMA R, MURARI A, et al. Oral candidiasis: an overview. J Oral Maxillofac Pathol,2014,18(Suppl 1): S81–S85.
    [12]
    METWALLI K H, KHAN S A, KROM B P, et al. Streptococcus mutans, Candida albicans, and the human mouth: a sticky situation. PLoS Pathog,2013,9(10): e1003616[2020-04-01]. https://doi.org/10.1371/journal.ppat.1003616.
    [13]
    SANGUINETTI M, POSTERARO B, LASS-FLÖRL C. Antifungal drugresistance among Candida species: mechanisms andclinical impact. Mycoses,2015,58(Suppl 2): 2–13.
    [14]
    DENG L, ZOU L, WU J, et al. Voriconazole inhibits cross-kingdom interactions between Candida albicans and Actinomyces viscosus through the ergosterol pathway. Int J Antimicrob Agents,2019,53(6): 805–813.
    [15]
    DENG L, LI W, HE Y, et al. Cross-kingdom interaction of Candida albicans and Actinomyces viscosus elevated cariogenic virulence. Arch Oral Biol,2019,100: 106–112.
    [16]
    ZHOU Y, LIAO M, ZHU C,et al. ERG3 and ERG11 genes are critical for the pathogenesis of Candida albicans during the oral mucosal infection.Int J Oral Sci, 2018, 10(2):9[2020-04-01].https://www.nature.com/articles/s41368-018-0013-2. doi: 10.1038/s41368-018-0013-2.
    [17]
    LIANG J, LI M, REN B, et al. The anti-caries effects of dental adhesive resin influenced by the position of functional groups in quaternary ammonium monomers. Dent Mater,2018,34(3): 400–411.
    [18]
    REN Z, CUI T, ZENG J, et al. Molecule targeting glucosyltransferase inhibits Streptococcus mutans biofilm formation and virulence. Antimicrob Agents Chemother,2015,60(1): 126–135.
    [19]
    MILLSOP J W, FAZEL N. Oral candidiasis. Clin Dermatol,2016,34(4): 487–494.
    [20]
    PEREIRA D, SENEVIRATNE C J, KOGA-ITO C Y, et al. Is the oral fungal pathogen Candida albicans a cariogen? Oral Dis,2018,24(4): 518–526.
    [21]
    PERSOON I F, CRIELAARD W, ZOK A R. Prevalence and nature of fungi in root canal infections: a systematic review and meta-analysis. Int Endod J,2017,50(11): 1055–1066.
    [22]
    XIAO J, HUANG X, ALKHERS N, et al. Candida albicans and early childhood caries: a systematic review and meta-analysis. Caries Res,2018,52(1/2): 102–112.
    [23]
    FALSETTA M L, KLEIN M I, COLONNE P M, et al. Symbiotic relationship between Streptococcus mutans and Candida albicans synergizes virulence of plaque biofilms in vivo. Infect Immun,2014,82(5): 1968–1981.
    [24]
    XIAO J, MOON Y, LI L, et al. Candida albicans carriage in children with severe early childhood caries (S-ECC) and maternal relatedness. PLoS One, 2016, 11(10): e0164242[2020-05-17]. https://doi.org/10.1371/journal.pone.0164242.
    [25]
    HWANG G, LIU Y, KIM D, et al. Candida albicans mannans mediate Streptococcus mutans exoenzyme GtfB binding to modulate cross-kingdom biofilm development in vivo. PLoS Pathog,2017,13(6): e1006407[2020-05-17].https://doi.org/10.1371/journal.ppat.1006407.
    [26]
    GREGOIRE S, XIAO J, SILVA B B, et al. Role of glucosyltransferase B in interactions of Candida albicans with Streptococcus mutans and with an experimental pellicle on hydroxyapatite surfaces. Appl Environ Microbiol,2011,77(18): 6357–6367.
    [27]
    BOWEN W H, KOO H. Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res,2011,45(1): 69–86.
    [28]
    ZENG L, BURNE R A. Comprehensive mutational analysisof sucrose-metabolizing pathways in Streptococcus mutans reveals novel roles for the sucrose phosphotransferasesystem permease. J Bacteriol,2013,195(4): 833–843.
    [29]
    HANS S, FATIMA Z, HAMEED S. Retrograde signaling disruption influences ABC superfamily transporter, ergosterol and chitin levels along with biofilm formation in Candida albicans. J Mycol Med,2019,29(3): 210–218.
    [30]
    LIU J F, XIA J J, NIE K L, et al. Outline of the biosynthesis and regulation of ergosterol in yeast.World J Microbiol Biotechnol, 2019, 35(7):98[2020-05-17].https://link.springer.com/article/ 10.1007/s11274-019-2673-2. doi: 10.1007/s11274-019-2673-2.
    [31]
    MISHRA N N, PRASAD T, SHARMA N, et al. Pathogenicity and drug resistance in Candida albicans and other yeast species. A review. Acta Microbiol Immunol Hung,2007,54(3): 201–235.
    [32]
    DANISVIJAY D, JAYANTHI S, MEENAKSHI N, et al. Characterization of virulence factors, antifungal resistance with ERG-11 gene among Candida species isolated from pulmonary samples. Microb Pathog,2019,137: 103750[2020-04-17].https://doi.org/10.1016/j.micpath.2019.103750.
    [33]
    VINCENT BM, LANCASTER AK, SCHERZ-SHOUVAL R, et al. Fitness Trade-Offs Restrict the Evolution of Resistance to Amphotericin B. PLoS Biol, 2013, 11(10):e1001692[2020-04-17]. https://doi.org/10.1371/journal.pbio.1001692.
    [34]
    SHAPIRO R S, ROBBINS N, COWEN L E. Regulatory circuitry governing fungal development, drug resistance, and disease. Microbiol Mol Biol Rev,2011,75(2): 213–267.
    [35]
    YI X, HUA Q, YAN W, et al. Requirement for ergosterol in berberine tolerance underlies synergism of fluconazole and berberine against fluconazole-resistant Candida albicans isolates. Front Cell Infect Microbiol,2017,7: 491[2020-04-17].https://doi.org/10.3389/fcimb.2017.00491.
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