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脉冲电磁场对兔膝骨关节炎软骨细胞凋亡及凋亡调控蛋白的影响
Pulsed Electromagnetic Field Therapy Inhibits Chondrocyte Apoptosis in Rabbits with Osteoarthritis
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摘要: 目的 探讨脉冲电磁场对兔膝骨关节炎软骨细胞凋亡及凋亡调控蛋白的影响。方法 将24只新西兰大白兔随机分为正常组、模型组和脉冲电磁场组，每组8只。除正常组外，其他两组动物均行右前交叉韧带切断术，术后6周，脉冲电磁场组用低频脉冲电磁场干预，每次40 min， 1次／d，每周5 d，共干预2周。干预结束后，取右股骨内外侧髁软骨进行HE染色（观察软骨全层结构和细胞形态）及Mankin评分、软骨细胞凋亡检测、凋亡调控蛋白（caspase-3和caspase-8）免疫组化检测。取右胫骨平台全层软骨提取总蛋白，采用Western blot检测caspase-3和caspase-8蛋白表达水平。结果 脉冲电磁场组Mankin评分高于正常组（P<0.01），但低于模型组（P<0.01）。脉冲电磁场组软骨细胞凋亡指数较正常组高（P<0.01）；但低于模型组（P<0.05）。免疫组化及Western blot检测：脉冲电磁场组caspase-3表达水平比正常组升高（P<0.01），但低于模型组（P<0.01）。脉冲电磁场组caspase-8表达水平比正常组升高（P<0.01），但与模型组比较，差异无统计学意义（P>0.05）。结论 脉冲电磁场能通过下调caspase-3表达的机制抑制软骨细胞凋亡，从而抑制兔膝骨关节炎关节软骨退变。Abstract: Objective?To determine the effect of pulsed electromagnetic field (PEMF) treatment on chondrocyte morphology, chondrocyte apoptosis, and the expression of apoptosis related proteins in rabbits. Methods?24 white New Zealand rabbits were randomly divided into three groups：normal control group (NC group), anterior cruciate ligament transection without treatment (ACLT group), and anterior cruciate ligament transection with pulsed electromagnetic field treatment (PEMF group). Six weeks after anterior cruciate ligament transection, the rabbits in the PEMF group were given 2 weeks of pulsed electromagnetic field treatment. Results?Rabbits in the PEMF group had significantly lower Mankin scores than those in the ACLT group, although the scores were higher than that of the NC group. The rabbits in the PEMF groups also had significantly lower levels of apoptosis index of chondrocytes and expression of caspase-3 compared with those in the ACLT group. The expression of caspase-8 in the rabbits in the PEMF group was higher compared to the NC group, but no significant difference compared with that of the ACLT group. Conclusion?Pulsed electromagnetic field treatment has therapeutic effect on the experimental osteoarthritis, which is likely a result of inhibition of apoptosis in chondrocytes.
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