miR-382-5p过表达介导PTEN的表达下调对人脑胶质瘤U251细胞恶性生物学行为的影响

赵树鹏; 姬颖华; 关庆凯; 马继伟; 靳彩玲; 王向阳; 赵新利

doi:10.12182/20200960502

miR-382-5p过表达介导PTEN的表达下调对人脑胶质瘤U251细胞恶性生物学行为的影响

Overexpression of miR-382-5p Overexpression Mediated Downregulation of PTEN on Malignant Biological Behavior of Human Glioma U251 Cells

摘要

摘要:
目的探究miR-382-5p过表达对人脑胶质瘤U251细胞恶性生物学行为的影响。
方法将miR-382-5p mimic转染入U251细胞，逆转录-聚合酶链反应检测miR-382-5p、磷酸酶-张力蛋白同源物（PTEN） mRNA水平；生物信息学预测miR-382-5p与PTEN存在碱基结合位点，构建PTEN pcDNA载体过表达，荧光素酶报告实验检测miR-382-5p与PTEN靶向关系，将细胞随机分为4组：Control组、mimics组、pc-PTEN组和mimics+pc-PTEN组进行后续实验，逆转录-聚合酶链反应检测各组细胞PTEN水平；克隆形成法检测细胞增殖；逆转录-聚合酶链反应检测Ki67、 Survivin、 c-Myc mRNA水平；Transwell检测细胞侵袭能力；蛋白免疫印迹检测E-cadherin、N-cadherin、Vimentin蛋白表达水平。
结果 miR-382-5p和PTEN存在直接靶向作用关系，与Control组相比较，mimics组miR-382-5p mRNA水平升高（P<0.05），PTEN mRNA水平降低（P<0.05），细胞克隆形成率降低（P<0.05），Ki67、Survivin mRNA水平降低（P<0.05），c-Myc mRNA水平升高（P<0.05），细胞侵袭数降低（P<0.05），E-cadherin蛋白水平升高（P<0.05），N-cadherin、Vimentin蛋白水平降低（P<0.05）。pc-PTEN组miR-382-5p mRNA水平降低（P<0.05），PTEN mRNA水平升高（P<0.05），细胞克隆形成率升高（P<0.05），Ki67、Survivin mRNA水平升高（P<0.05），c-Myc mRNA水平降低（P<0.05），细胞侵袭数升高（P<0.05），E-cadherin蛋白水平降低（P<0.05），N-cadherin、Vimentin蛋白水平升高（P<0.05）；与pc-PTEN组相比较，mimics+pc-PTEN组miR-382-5p mRNA水平升高（P<0.05），PTEN mRNA水平降低（P<0.05），细胞克隆形成率降低（P<0.05），Ki67、Survivin mRNA水平降低（P<0.05），c-Myc mRNA水平升高（P<0.05），细胞侵袭数降低（P<0.05），E-cadherin蛋白水平升高（P<0.05），N-cadherin、Vimentin蛋白水平降低（P<0.05）。
结论 miR-382-5p过表达介导PTEN的表达下调可抑制胶质瘤U251细胞增殖、侵袭、生长和上皮细胞-间充质转化。

Abstract:
Objective To investigate the effect of overexpression of miR-382-5p overexpression on malignant biological behavior of human glioma U251 cells.
Methods U251 cells were transfected with miR-382-5pmimic. Then miR-382-5p and PTEN mRNA levels were detected by reverse transcription-polymerase chain reaction (RT-PCR) after transfection. Used bioinformatics to predicted the presence of base binding sites between miR-382-5p and PTEN, and constructed PTEN pcDNA vector overexpression plasmid was constructed. Luciluciase reporting experiment was used to detect the targeting relationship between miR-382-5p and PTEN. Cells were randomly divided into four groups: control group, mimics group, pc-PTEN group and mimics+pc-PTEN group for follow-up experiments. RT-PCR was carried out to detect the level of PTEN mRNA in each group. Cell proliferation was detected by clone formation method. The mRNA levels of Ki67, Survivin and c-Myc were detected by RT-PCR. Transwell experiment was used to assayed cell invasion ability. The expression levels of E-cadherin, N-cadherin and Vimentin were determined by Western blot.
Results Results showed that miR-382-5p directly targeted PTEN. Compared with the control group, miR-382-5p and c-Myc mRNA levels and E-cadherin protein level were increased (P<0.05), PTEN, Ki67 and Survivin mRNA levels were decreased (P<0.05), cell clonal formation rate and cell invasion number were decreased (P<0.05), N-cadherin and Vimentin protein levels were decreased (P<0.05) in the mimics group; In pc-PTEN group, miR-382-5p mRNA and c-Myc mRNA levels and E-cadherin protein level were decreased (P<0.05), PTEN, Ki67 and Survivin mRNA levels were increased (P<0.05), cell clonal formation rate and cell invasion number were increased (P<0.05), N-cadherin and Vimentin protein levels were increased (P<0.05). Compared with pc-PTEN group, PTEN, Ki67 and Survivin mRNA levels, the cell clone formation rate, the number of invasion cells and the N-cadherin and Vimentin levels of mimics+PC-PTEN group decreased significantly (P<0.05), while the c-Myc mRNA level and E-cadherin protein level increased significantly (P<0.05).
Conclusion Overexpression of miR-382-5p mediates the downregulation of PTEN expression, causing the inhibition of the proliferation, invasion, growth and EMT of U251 glioma cells.

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