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谷沛霖, 沈建磊, 诸颖, 等. 组织透明化技术的发展及其在神经退行性疾病中的应用[J]. 四川大学学报(医学版), 2021, 52(3): 350-356. DOI: 10.12182/20210560302
引用本文: 谷沛霖, 沈建磊, 诸颖, 等. 组织透明化技术的发展及其在神经退行性疾病中的应用[J]. 四川大学学报(医学版), 2021, 52(3): 350-356. DOI: 10.12182/20210560302
GU Pei-lin, SHEN Jian-lei, ZHU Ying, et al. Development in Tissue Clearing Technology and Its Application in Neurodegenerative Diseases[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(3): 350-356. DOI: 10.12182/20210560302
Citation: GU Pei-lin, SHEN Jian-lei, ZHU Ying, et al. Development in Tissue Clearing Technology and Its Application in Neurodegenerative Diseases[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(3): 350-356. DOI: 10.12182/20210560302

组织透明化技术的发展及其在神经退行性疾病中的应用

Development in Tissue Clearing Technology and Its Application in Neurodegenerative Diseases

  • 摘要: 现代组织透明化技术使细胞群的高分辨率成像和组织结构的三维重建成为可能,我们通过组织透明化技术可以获得更完整的脑部三维结构以及脑组织各个组分的空间联系。在过去的十年里,科学家们对组织透明化技术进行了大量的开发与改进,这些方法目前被广泛的运用在神经科学的研究中,使我们在复杂的神经网络中得到重要的信息。同时,组织透明化技术也会为神经退行性疾病的干细胞治疗和神经再生提供研究工具。在这篇综述中,我们主要回顾了一下目前主要的组织透明化技术的种类以及优缺点,并且总结了一下这些技术在神经退行性疾病研究中的应用以及独特的优势,此外我们还探讨了未来组织透明化技术的发展要求,配套设备的完善以及在干细胞治疗和再生医学中作为研究工具的潜力。

     

    Abstract: Modern tissue clearing techniques have made it possible to have high-resolution imaging of cell populations and three-dimensional reconstruction of tissue structures, and we are able to obtain more complete three-dimensional brain structures and spatial connections between the various components of brain tissues through tissue clearing techniques. Over the past decade, scientists have developed and improved a number of tissue clearing techniques that are now widely used in neuroscience research, allowing us to extract important information from complex neural networks. Moreover, tissue clearing technology also provides research tools for the stem cell therapy and neurogeneration of neurodegenerative diseases. In this paper, we reviewed the major types of existing tissue clearing techniques and their respective strengths and weaknesses. We summarized the application of these techniques in neurodegenerative disease research and their unique merits. In addition, we explored the development requirements of tissue clearing technology, improvements in the supporting equipment, and its potential to be used as research tools for stem cell therapy and regenerative medicine in the future.

     

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