芦荟大黄素金属配合物的抗氧化活性研究
Study on the Antioxidant Activity of Aloe-Emodin Metal Complex
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摘要:目的 合成3种芦荟大黄素金属配合物,并比较配体和配合物的抗氧化活性。方法 在无水乙醇溶剂中,通过溶解、搅拌合成芦荟大黄素-铁(Ⅱ)、芦荟大黄素-铜(Ⅱ)、芦荟大黄素-镁(Ⅱ)3种配合物,并对其结构进行表征,采用Fe2+-H2O2-亚甲蓝法、二苯代苦味肼基自由基法(DPPH法)等检测配体和配合物对超氧自由基(O2-•)、羟基自由基(•OH)、二苯代苦味肼基自由基(DPPH•)的清除作用。结果 成功合成芦荟大黄素-铁(Ⅱ)、芦荟大黄素-铜(Ⅱ)、芦荟大黄素-镁(Ⅱ)3种配合物,根据结构表征结果,推测芦荟大黄素金属配合物可能结构为两分子芦荟大黄素通过9位羰基和8位羟基与一分子金属离子(Fe2+、Mg2+、Cu2+)形成配合物。配合物和配体对3种自由基均有清除作用,但配合物的清除作用强于其配体(P<0.05)。结论 芦荟大黄素与Fe2+、Cu2+、Mg2+ 3种金属离子配位后,增强了其配体本身的抗氧化活性。Abstract:Objective To synthesize three kinds of metal complexes of aloe-emodin and compare the antioxidant activities of the ligands and the complexes.Methods Three kinds of aloe emodin metal complex, the aloe-emodin-iron (Ⅱ), the aloe-emodin-copper (Ⅱ) and the aloe-emodin-magnesium (Ⅱ) complexes, were synthesized by dissolving and stirring in anhydrous ethanol solvent, and their structures were characterized. The Fe2+-H2O2-methylene blue method, the diphenyl bitter hydrazine radical method (DPPH method) and other assays were used to determine the clearance effect of ligands and complexes on superoxide radicals (O2-•), hydroxyl radicals (•OH) and phenyl bitter hydrazine radical (DPPH•).Results Three kinds of aloe emodin metal complex, the aloe-emodin-iron (Ⅱ), the aloe-emodin-copper (Ⅱ) and the aloe-emodin-magnesium (Ⅱ) complexes, were successfully synthesized. According to the results of structural characterization, we speculated that the aloe-emodin metal complexes were formed at the site between the two molecules of aloe-emodin and one molecule of metal ions (Fe2+, Mg2+, Cu2+) via the 9th carbonyl and 8th hydroxyl groups of the aloe-emodin molecules. Both the complex and the ligand have clearance effects on three kinds of free radicals, and the complex showed stronger effects than its ligand (P<0.05).Conclusion Coordination of aloe-emodin with metal ions, such as Fe2+, Cu2+, and Mg2+, could enhance the antioxidant activity of the ligand itself.
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