卵泡抑素相关基因在结肠癌中的表达及其临床意义

何雪铼; 熊海波; 侯能易; 罗仁杰; 余松; 胡雪梅; 许杰; 庞明辉

doi:10.12182/20200560104

卵泡抑素相关基因在结肠癌中的表达及其临床意义

Expression Level of FLRG in Colon Cancer Tissue and Its Clinical Significance

摘要

摘要:
目的探讨卵泡抑素相关基因（follistatin related-gene, FLRG）在结肠癌中的表达及其与结肠癌临床病理特征的关系。
方法选取2018年12月—2019年12月收治的行根治性手术的结肠癌患者的肿瘤组织、癌旁及正常组织各80例，采用免疫组织化学染色及实时荧光定量PCR方法检测FLRG的表达，并分析其表达水平与结肠癌病理特征的关系。
结果 FLRG在结肠癌组织中的表达高于癌旁组织及正常组织，在癌旁组织中的表达高于正常组织，差异均有统计学意义（P<0.05）。FLRG的表达在结肠癌患者不同性别、不同年龄、不同肿瘤生长位置及分化程度间无明显差异（P>0.05），结肠癌伴远处转移的患者FLRG表达水平高于无远处转移患者（P<0.05），临床分期较晚的患者FLRG表达水平高于临床分期较早的患者（P<0.05）。
结论 FLRG在结肠癌组织中表达上调，可能参与肿瘤发生发展的调控，是潜在的预后靶点。

Abstract:
Objective To investigate the expression of follistatin related gene (FLRG) in colon cancer and its relationship with clinicopathological features of colon cancer.
Methods The cancer tissue, paracancerous tissue and normal tissue were collected from 80 patients with colon cancer who underwent radical operation from December 2018 to December 2019. Immunohistochemistry and Real-time PCR were carried out to examine the expression of FLRG and the clinical implications of FLRG was further analyzed.
Results The expression of FLRG in colon cancer tissues was significantly higher than that in paracancerous tissues and normal tissues (P<0.05), and the expression of FLRG in paracancerous tissues was significantly higher than that in normal tissues (P<0.05). There was no significant difference in the expression of FLRG among colon cancer patients with different sex, age, tumor growth location and differentiation degree (P>0.05). The expression level of FLRG in patients with distant metastasis was higher than that in patients without distant metastasis (P<0.05), and the expression level of FLRG in patients with late clinical stage (stage Ⅲ and Ⅳ) was higher than that in patients with earlier clinical stage (stage Ⅰ and Ⅱ) (P<0.05).
Conclusion FLRG is up-regulated in colon cancer tissue, which may be involved in the regulation of tumor development. FLRG may be a potential prognostic target.

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