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李聪伟, 李倩, 郭兰等. 芦丁对1型糖尿病小鼠肝功能及肝组织形态学的影响[J]. 四川大学学报(医学版), 2018, 49(3): 384-387.
引用本文: 李聪伟, 李倩, 郭兰等. 芦丁对1型糖尿病小鼠肝功能及肝组织形态学的影响[J]. 四川大学学报(医学版), 2018, 49(3): 384-387.
LI Cong-wei, LI Qian, GUO Lan. et al. Effect of Rutin on Liver Function and Morphology in Type 1 Diabetes Mice Induced by Streptozotocin[J]. Journal of Sichuan University (Medical Sciences), 2018, 49(3): 384-387.
Citation: LI Cong-wei, LI Qian, GUO Lan. et al. Effect of Rutin on Liver Function and Morphology in Type 1 Diabetes Mice Induced by Streptozotocin[J]. Journal of Sichuan University (Medical Sciences), 2018, 49(3): 384-387.

芦丁对1型糖尿病小鼠肝功能及肝组织形态学的影响

Effect of Rutin on Liver Function and Morphology in Type 1 Diabetes Mice Induced by Streptozotocin

  • 摘要: 目的 探究芦丁对链脲佐菌素(STZ)诱导的1型糖尿病小鼠肝功能及肝脏组织形态的影响。方法 雄性昆明小鼠60只,选出12只作为正常组,余下小鼠腹腔注射STZ,连续给药5 d,建立1型糖尿病小鼠模型。成模小鼠随机划分为模型组与芦丁低、高剂量组。正常组与模型组给予羧甲基纤维素钠溶液, 芦丁低、高剂量组分别按小鼠体质量予芦丁50 mg/kg、100 mg/kg灌胃,给药8周,每日1次。测定给药第4周,第8周小鼠餐后随机血糖,血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、总蛋白(TP)和白蛋白(ALB)的含量。通过HE、Masson染色及透射电镜观察肝组织形态。结果 与正常组比,模型组小鼠血糖上升(P<0.01),血清中ALT、AST、LDH含量增高,TP、ALB含量降低(P<0.05,P<0.01);与模型组比,芦丁组小鼠给药第4周血糖降低(P<0.05),给药第8周血清ALT、AST、LDH含量下降,TP、ALB含量升高,其中高剂量组作用更明显(P<0.05,P<0.01)。形态学结果显示,模型组肝细胞增大变形,肝索排列紊乱,肝组织纤维化程度较高;芦丁低、高剂量组小鼠肝组织形态有所改善,高剂量组作用更明显。结论 芦丁可改善STZ诱导的1型糖尿病小鼠的肝功能,缓解糖尿病小鼠肝脏组织的损伤程度。

     

    Abstract: Objective To investigate the effect of Rutin on the function and morphology of liver in type 1 diabetes mice induced by streptozotocin (STZ). Methods Type 1 diabetic mice model was established with the treatment of STZ by a 5 d intraperitoneal administration to male Kunming mice. Normal group had 12 mice without treatment of STZ, the mice with DM after STZ treated were randomly divided into DM group, low-dose(50 mg/kg) Rutin group and high-dose (100 mg/kg) Rutin group, each group had 12 mice. The mice in normal and DM group were given sodium carboxymethyl cellulose (10 mg/kg). Each mice was given above drugs by intragastric administration for 8 weeks. Postprandial random blood glucose was measured at 4 weeks and 8 weeks and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total protein (TP) and albumin (ALB) in serum were detected by automatic biochemical analyzer after 8 weeks. The morphology of liver was observed by HE and Masson staining. The ultrastructure of liver tissue was observed by electron microscope. Results After a continuous small-dose injection of STZ, the success rate of diabetes model mice were up to 98%. The blood glucose of the model group was significantly increased (P<0.01), and the levels of ALT, AST and LDH in serum were significantly higher, and TP and ALB were lower than those in normal group (P<0.05, P<0.01). Compared to the DM group, the levels of blood glucose were lower (P<0.05) at 4 weeks and 8 weeks, the contents of ALT, AST and LDH were significantly decreased, and TP and ALB were improved in both Rutin dose group. High-dose group performed more obvious (P<0.05, P<0.01). Morphological observation showed the tissue morphology of Rutin treatment group were improved obviously, and the effect was more significant in high-dose Rutin group. Conclusion Rutin may improve the liver function and reduce the damage of liver tissue in STZ-induced type 1 diabetic mice.

     

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