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陈明凯, 欧梦婵, 郝学超, 等. 新生大鼠长时间暴露于七氟烷对注意缺陷多动障碍的影响[J]. 四川大学学报(医学版), 2021, 52(2): 207-215. DOI: 10.12182/20210360601
引用本文: 陈明凯, 欧梦婵, 郝学超, 等. 新生大鼠长时间暴露于七氟烷对注意缺陷多动障碍的影响[J]. 四川大学学报(医学版), 2021, 52(2): 207-215. DOI: 10.12182/20210360601
CHEN Ming-kai, OU Meng-chan, HAO Xue-chao, et al. Effect of Long-time Postnatal Exposure to Sevoflurane on Causing Attention-deficit/Hyperactivity Disorder in Rats[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(2): 207-215. DOI: 10.12182/20210360601
Citation: CHEN Ming-kai, OU Meng-chan, HAO Xue-chao, et al. Effect of Long-time Postnatal Exposure to Sevoflurane on Causing Attention-deficit/Hyperactivity Disorder in Rats[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(2): 207-215. DOI: 10.12182/20210360601

新生大鼠长时间暴露于七氟烷对注意缺陷多动障碍的影响

Effect of Long-time Postnatal Exposure to Sevoflurane on Causing Attention-deficit/Hyperactivity Disorder in Rats

  • 摘要:
      目的  探讨新生大鼠长时间吸入七氟烷是否导致注意缺陷多动障碍(attention-deficit/hyperactivity disorder,ADHD)相关的反常活动和记忆损害。
      方法  将出生后5 d(postnatal day 5,P5)的SD大鼠随机分为七氟烷组和对照组,分别采用吸入3%的七氟烷和吸入相同流量纯氧2 h、4 h建立七氟烷吸入麻醉模型及处理对照组。采用旷场实验(open field test,OFT)、5项选择连续反应时间任务实验(5-choice serial reaction time task,5-CSRTT)、场景恐惧(fear-conditioning,FC)实验、水迷宫(morris water maze,MWM)实验评估青少年期大鼠(~P25)和成年大鼠(~P65)的认知、记忆、焦虑及ADHD相关行为等变化。
      结果  在OFT中,七氟烷2 h、4 h组大鼠P21、P61活动水平和探索行为指标与对照组的差异均无统计学意义;5-CSRTT实验结果显示,七氟烷组在P25和P65时ADHD相关行为(不成熟反应次数、正确率、遗漏率)与对照组比较,差异也无统计学意义;在FC实验中,与对照组相比,七氟烷4 h暴露组大鼠僵直时间缩短,僵直潜伏期时间延长(P=0.029);MWM实验结果显示,与对照组相比,七氟烷4 h组大鼠训练过程中逃逸潜伏期在第二天和第三天延长(P<0.05),七氟烷组平均游泳速度在P69和P76时差异无统计学意义,七氟烷4 h组的目标象限停留时间缩短(P=0.039),目标象限移动距离百分比降低(P=0.048)。
      结论  新生大鼠七氟烷吸入麻醉4 h后,可致记忆损害但并未增加发生ADHD相关反常活动的风险。

     

    Abstract:
      Objective   To investigate whether long-term exposure to inhaled sevoflurane, a volatile anesthetic, causes abnormal activities and memory impairment related to attention-deficit/hyperactivity disorder (ADHD) in neonatal rats.
      Methods  On postnatal day 5 (P5), Sprague-Dawley rats were randomly assigned to two sevoflurane subgroups and two control subgroups and underwent experimental intervention. The two sevoflurane (SEVO) subgroups were exposed to 3% sevoflurane for 2 h and 4 h respectively, while the two control subgroups were given pure oxygen for the same amount and duration. Behavioral tests, including open-field test (OFT), five-choice serial reaction time task (5-CSRTT), fear-conditioning (FC) and Morris water maze (MWM), were applied to evaluate changes in cognition, memory, anxiety and ADHD-related behavioral changes in the rats in adolescence (-P25) and in adulthood (-P65).
      Results  In OFT, the SEVO 2 h and SEVO 4 h subgroups displayed activity level and exploratory behaviors similar to those of the control subgroups on P21 and P61, with no statistically significant difference identified in the data. 5-CSRTT results on P25 and P65 indicated no statistically significant difference between the SEVO subgroups and the control subgroups in regard to ADHD-related abnormal behaviors, including number of immature reaction, rate of correct response and omission rate. In the FC experiment, SEVO 4 h group had a shorter freezing period and longer period of freezing latency (P=0.029) in comparison to the control groups. The results of the MWM test showed that the escape latency period of rats in the SEVO 4 h group was significantly prolonged on the second day and the third day, compared to the control groups (P<0.05). The average swimming speed of SEVO groups did no exhibit any statistically significant difference on P69 or P76. The time the SEVO 4 h group spent in the target quadrant was significantly shorter than that of the control group (P=0.039) and percentage of distance traveled in the target quadrant was significantly reduced compared to that the control group (P=0.048).
      Conclusion  The findings suggest that four hours of inhaled sevoflurane exposure in neonate rats may cause memory impairment, but does no increase risks for ADHD-related abnormal activities.

     

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